2017 Schedule of Events

June 6-7th 2017

The tentative schedule is listed below within separate sections:

I ) Symposium 
II) Equipment, software and technical demonstrations
III) Hands-on practicals 

I) Symposium Schedule

Visualizing cell communication by cryo-EM techniques  
TAC building auditorium N107  
Tuesday afternoon, June 6th
12:00–1:00 PM"4D electron microscopy with millisecond temporal resolution" 
Shigeki Watanabe, PhD 
Depts of Cell Biology and Neuroscience 
Johns Hopkins University, MD
Image Analysis 
TAC building auditorium N107 
Tuesday afternoon, June 6th 
1:00–2:00 PM"CellProfiler: free, advanced image analysis software for microscopy and imaging flow cytometry"
Anne Carpenter, PhD
Imaging Platform Center
Broad Institute of Harvard and MIT
2:00–3:00 PM"Inferring causal relations in molecular pathways from live cell images"
Gaudenz Danuser, PhD
Dept of Bioinformatics
University of Texas Southwestern Medical Center
Coffee break
3:30-4:00"Cryo-EM of membrane protein in a membrane: Kv1.2 in liposomes"  
Fred Sigworth, PhD  
Dept of Molecular and Cellular Physiology 
Yale University
4:00–5:00 PM"Segmentation and analysis of multidimensional EM datasets using Microscopy Image Browser
Eija Jokitalo, PhD 
Institute of Biotechnology
University of Helsinki, Finland
Happy Hour to follow
  
High-dimensional imaging
TAC building auditorium N107
Wednesday afternoon, June 7th
1:00-2:00 PM"miCAT image cytometry analysis toolbox for the systematic analysis of cellular networks in highly multiplexed tissue images."  
Denis Schapiro, PhD  
Institute of Molecular Life Sciences 
University of Zurich, Switzerland 
2:00–3:00 PM"Quantitative cell subsetting by morphology using Amnis imaging flow cytometry"
Kathleen McGrath, PhD
Center for Pediatric Biomedical Disease
University of Rochester, NY
Coffee break

 

3:30-4:30 PM"Highly multiplexed fluorescent imaging by simplified antibody stripping for 18+ channel staining" 
Giorgio Cattoretti, MD 
Dept of Medicine 
Universata degli Studi di Milano-Biococca
Happy Hour to follow

II) Equipment, Software and Technical Demonstrations and Lectures

Tuesday, June 6th
9:00–5:00 PM Software Walk-in Clinics for post acquisition image analysis (except during relevant lectures)
Imaris (9-5pm - TAC N213), Amira (11-5pm, Tues only - TAC N209), In Cell Analyzer (9-5pm - TAC N237), CellProfiler Analyst (9:30-5, Tues only - TAC N207), miCAT (10-12:30 on Tues - TAC N211)  
9:00–5:00 PM Continued access to most participating instruments throughout the day
See the Equipment page for locations on campus and the FAQs page for information on reservations
9:00–9:30 AM Demonstration of FEI Vitrobot for cryo sample preparation 
Staff demonstration within Yale EM facility - SHM IE-26
9:30–10:30 AM "Correlative light and Cryo electron microscopy"
Louise Bertrand, Leica lecture and demo of cryo CLEM - TAC N223 
10:00–10:30 AM "Environmental considerations for live-cell imaging"  
 Dan Focht Bioptechs technical lecture, followed by demonstration - TAC N203, TAC N229
10:30–11:00 AM "Detecting and tracking objects in Amira for cell biology"  
Trevor Lancon, Amira - TAC N203
10:30–11:30 AM Demonstration of cryo-prepared sample TEM using FEI Technai F20 
Staff demonstration within Yale EM facility - SHM IE-26
11:00-11:30 AM Demo of Nikon Eclipse Ti2 features for high-throughput and large field of view  
Nikon demonstration in TAC N229
11:30–12:15 PM "Deconvolving microscopy and high-throughput imaging analysis"  
Damien McCann, GE technical lecture and demonstration - TAC N237
11:30–12:15 PM "LSM 880-Airyscan - improving resolution and signal-to-noise in cryo-fluorescence microscopy as part of a correlative EM-LM workflow"   
Zeiss technical lecture and demonstration - TAC N231 
12:30–1:00 PM
"3D/4D image visualization and analysis with Imaris 8" 
Brian Thompson, Imaris technical lecture - TAC N221
1:00 - 2:00 PM  Demonstration of Leica ICE system for high pressure freezing with integrated stimulation  
Leica demo - SHM IE40
Wednesday, June 7th
9:00–5:00 PM Software Walk-in Clinics for post acquisition image analysis (except during relevant lectures) 
Imaris (9-5pm - TAC N213), GE InCell Analyzer (9-5pm - TAC N237), miCAT (9-11 Wed - TAC N211), MIB (9-12 Wed only - TAC N207)
9:00–5:00 PM Continued access to most participating instruments throughout the day
See the Equipment page for locations on campus and the FAQs page for information on reservations
9:30–10:30 AM "Cryo EM tomography"
FEI technical lecture in TAC N203 
9:30–10:30 AM Demonstration of Leica Cryo-CLEM microscope and accessories 
Leica demo - TAC N223
10:30-11:00 AM "Super-resolution imaging with Leica STED3X"    
Sarah Crowe, Leica - TAC N203
11:00-12:00 PM Demonstration of cryo-prepared sample TEM using FEI Technai F20  
Staff demonstration within Yale EM facility - SHM IE-26
11:00–11:45 AM Demonstration of Lavision BioTec two-photon laser scanning microscope
Staff demonstration in TAC S614
11:30–12:15 PM "Abberior Instruments STEDYCON - a new class of microscopes" 
Karsten Bahlmann, Abberior - TAC N237
12:00–1:00 PM "Amnis Imagestream imaging flow cytometry applications" 
Amnis - TAC N22112:00–1:00 PM
12:00–12:30 PM Demonstration of Zeiss CellDiscoverer 7 
Zeiss in TAC S231

III) Practical Micro-Courses

Space is limited in these micro-courses! Advance registration is required.
Tuesday, June 6th
9:30 AM - 10:30AM Small group practical session on high-throughput imaging
(for group #1) at participating HT widefields in the North TAC wing  
10:30 AM - 11:30AM Small group practical session on imaging flow cytometry
(for group #2) at the Amnis Imagestream MkII in TAC S613 
1:00–5:00 PM Opportunity for individual practice - please reserve an instrument through the online scheduling system for guided use
   
Wednesday, June 7th
9:30 AM - 10:30AM Small group practical session on high-throughput imaging
(for group #3) at participating HT widefields in the North TAC wing
10:30 AM - 11:30AM Small group practical session on imaging flow cytometry
(for group #4) at the Amnis Imagestream MkII in TAC S613 
1:00–5:00 PM Opportunity for individual practice - please reserve an instrument through the online scheduling system for guided use