Schedules of Events
Listed below within separate sections
I ) symposium
II) lecture and practicals
III) equipment and software demos
I) Symposium Schedule
TAC building auditorium N107
Tuesday June 10th FRET FOCUS
9:30 – 10:30 AM “Dissection Ca2+ and phosphoinositide signaling pathways using chemical perturbations
and live-cell imaging”
Tobias Meyer, Ph.D.
Department of Chemical and Systems Biology
Stanford University
Coffee break
11:00 –12:00 "Stimulated calcium dynamics in mast cells: space, time, and molecular interactions"
Barbara Baird, Ph.D.
Division of Chemistry and Chemical Biology
Cornell University
Wednesday June 11th 3D AND 4D IMAGING
9:30 – 10:30 AM “3D and 4D imaging of zebrafish visual system structure and development”
Chi-Bin Chien, Ph.D.
Department of Neurobiology and Anatomy
University of Utah Medical Center
Coffee break
11:00 – 12:00 “From SAP-less T cells to helpless B cells and back: 2-photon illumination of
dynamic cell cooperation underlying the formation of germinal centers”
Hai Qi, Ph.D.
Division of Immunology
NIH, NIAID
------------------------
II) Classes, Hands-on Practicals, and Technical Demonstrations
Tuesday June 10th
1:00 – 2:00 PM "Molecular imaging using FRET microscopy: a technical lecture and application
in innate immunity research"
Eicke Latz, M.D. Ph.D.
Division of Infectious Diseases and Immunology
University of Massachusetts Medical School
TAC building room N203
2:00 – 4:00 PM FRET hands-on practical
with confocals from all major vendors
TAC N221 - N229
4:00 – 5:00 PM TIRF Demonstration
Olympus multi-angle and color TIRF TAC N223
4:00 – 5:00 PM Approaches to Live Cell Imaging
Nikon demo of Biostation IM, A1 confocal and TIRF TAC N221
Wednesday June 11th
1:00 – 2:00 PM "Approaches to optical sectioning of tissue: confocal and two-photon
laser scanning microscopy and other emerging techniques"
Michael Levene, Ph.D.
Division of Biomedical Engineering
Yale University
TAC auditorium N107
2:00 – 4:00 PM Optical sectioning hands-on practical
thick tissue samples provided as well as fixed cell slides
TAC N221 - N229
4:00 – 5:00 PM 3D rendering and deconvolution practical
post-acquisition image analysis
Imaris TAC N207
4:00 – 5:00 PM Structured Illumination for Widefield Microscopy
Zeiss Apotome demonstration TAC N229
4:00 – 5:00 PM Laser Microdissection Demonstration
Leica LMD demonstration TAC N231
Thursday June 12th
11:00 – 12:00 Fluorescence Lifetime Imaging (FLIM) Demonstration
LaVision Biotec TAC N237
1:00 – 3:00 PM FRET hands-on practical (2nd cohort for Yale personnel only)
with confocals from all major vendors
TAC N225 - N229
1:00 – 3:00 PM Optical sectioning hands-on practical (2nd cohort for Yale personnel only)
thick tissue samples provided as well as fixed cell slides
TAC N221 - N223
4:00 – 5:00 PM 3D rendering and deconvolution practical
post-acquisition image analysis
Imaris TAC N207
------------------------
III) Equipment and Software Demonstrations
Tuesday June 10th
10:00 – 4:00 PM Software Walk-in Clinics for post acquisition image analysis
Imaris (TAC N207)
9:00 – 2:00 PM Continued access to all instrumentation througout the day
TAC N221 - N229
Wednesday June 11th
10:00 – 4:00 PM Software Walk-in Clinics for post acquisition image analysis
Imaris (TAC N207)
9:00 – 2:00 PM Continued access to all instrumentation througout the day
TAC N2221 - N229
Thursday June 12th
9:00 – 2:00 PM Continued access to all instrumentation througout the day
TAC N2221 - N229
10:00 – 4:00 PM Software Walk-in Clinics for post acquisition image analysis
Imaris (TAC N207)