
Schedules of Events
Listed below within separate sections
I ) symposium
II) lecture and practicals
III) equipment and software demos
I) Symposium Schedule
TAC building auditorium N107
Tuesday June 9th Imaging Development - Part I
2:00 – 2:30 PM “Cytoskeletal Dynamics During Drosophila Oogenesis”
Lynn Cooley, Ph.D.
Department of Genetics
Yale University
2:30 – 3:30 PM “Imaging Stem Cells and Cell Lineage in Drosophila”
Allan Spradling , Ph.D.
Department of Embyrology
Carnegie Institution
Coffee break
Super Resolution Approaches
4:00 – 5:00 PM “Three Dimensional Superresolution Microscopy of Focal Adhesions”
Pakorn Kanachanawong , Ph.D.
Laboratory of Cell and Tissue Morphodynamics
NIH/NHLBI
Wednesday June 10th Imaging Development - Part II
2:00 – 2:30 PM “Imaging Axon Regeneration In Vivo”
Marc Hammarlund, Ph.D.
Department of Genetics
Yale University
2:30 – 3:30 PM “Imaging the Dynamics of Embryonic Development”
Scott Fraser , Ph.D.
Department of Applied Physics
California Institute of Technology
Coffee break
4:00 – 5:00 PM “Multidimensional visualization of zebrafish embryogenesis by
multidirectional selective plane illumination microscopy (mSPIM)”
Jan Huisken , Ph.D.
Department of Biochemistry and Biophysics
University of California, San Francisco
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II) Classes, Hands-on Practicals, and Technical Demonstrations
Tuesday June 9th
11:00 – 12:00 Spinning Disk Demonstration
Perkin Elmer UltraView Vox demonstration TAC N239
12:00 – 1:30 " Time Correlated Single Photon Counting : use for FLIM, FRET and FCS"
lecture in TAC N207
followed by FLIM demo in TAC N237
Thomas Pingle , LaVision Biotec
1:00 – 2:00 PM Laser Microdissection Demonstration
Leica LMD demonstration TAC N221
1:00 – 2:00 PM "Getting closer to reality through deconvolution: the most
payout for the photons you put in"
lecture on deconvolution in TAC N211
Jeff M. Reece , Reecent Technologies/ Scientific Volume Imaging
Wednesday June 10th
9:30 – 10:30 AM "Choosing imaging parameters for time resolved imaging of live samples
for cell tracking" (advance registration for this lecture is strictly required!)
Bill Mohler , Ph.D.
Department of Genetics and Developmental Biology
UConn Health Center
TAC building auditorium N107
10:30–12:00 AM Hands-on practical in small groups: 4D imaging of fluorescent Drosophila
embryos with confocal or two-photon laser scanning microscopes (for group #1)
TAC N221 - N229 Registration for this class is now closed. The class is full.
11:00–12:00 AM Demo of Laser Scanning Cytometry for High Content Screening
CompuCyte iCys demonstration TAC N239
1:00 – 2:00 PM Small group break--out for post acquisition analysis using Volocity, Imaris,
or MetaMorph software (for group #1)
TAC N207 - N211 Registration for this class is now closed. The class is full.
2:00 – 5:00 PM Opportunity for individual practice - please reseve an instrument for personal use
Thursday June 11th
10:30–12:00 AM Hands-on practical in small groups: 4D imaging of fluorescent Drosophila
embryos with confocal or two-photon laser scanning microscopes (for group #2)
TAC N221 - N229 Registration for this class is now closed. The class is full.
1:00 – 2:00 PM Small group break--out for post acquisition analysis using Volocity, Imaris,
or MetaMorph software (for group #2)
TAC N207 - N211 Registration for this class is now closed. The class is full.
2:00 – 5:00 PM Opportunity for individual practice - please reseve an instrument for personal use
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III) Equipment and Software Demonstrations
Tuesday June 9th
Software Walk-in Clinics for post acquisition image analysis
Imaris (TAC N209) 9 AM - 5 PM
MetaMorph (TAC N207) 1 - 5 PM
Huygens (TAC N211) 9 AM - 5 PM
Volocity (TAC N239) Note: only available from 1-3 PM
9:00 – 5:00 PM Continued access to most instrumentation throughout the day
TAC N221 - N239
Wednesday June 10th
2:00 – 5:00 PM Software Walk-in Clinics for post acquisition image analysis
Imaris (TAC N209) 9 AM - 5 PM
MetaMorph (TAC N207) 9 AM - 5 PM
Huygens (TAC N211) 9 AM - 3:30 PM
Volocity (TAC N239) Note: only available from 1-3 PM
9:00 –5:00 PM Continued access to most instrumentation throughout the day
TAC N2221 - N239
Thursday June 11th
2:00 – 5:00 PM Software Walk-in Clinics for post acquisition image analysis
(Imaris is not available on this day)
MetaMorph (TAC N209) 9 AM - 5 PM
Huygens (TAC N211) 9 AM - 5 PM
Volocity (TAC N239) Note: only available from 1-3 PM
9:00 –5:00 PM Continued access to most instrumentation throughout the day
TAC N2221 - N239