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Schedules of Events

Listed below within separate sections
     I ) symposium
     II) lecture and practicals
     III) equipment and software demos

I) Symposium Schedule
    TAC building auditorium N107
   Tuesday June 9th Imaging Development - Part I

   
    2:00 – 2:30 PM    “Cytoskeletal Dynamics During Drosophila Oogenesis”
                                    Lynn Cooley, Ph.D.
                                    Department of Genetics
                                    Yale University
    
    2:30 – 3:30 PM   Imaging Stem Cells and Cell Lineage in Drosophila”
                                  
  Allan Spradling , Ph.D.
                                    Department of Embyrology
                                    Carnegie Institution  
    Coffee break
    
   Super Resolution Approaches

    4:00 – 5:00 PM    “Three Dimensional Superresolution Microscopy of Focal Adhesions”
                                    Pakorn Kanachanawong , Ph.D.
                                    Laboratory of Cell and Tissue Morphodynamics
                                    NIH/NHLBI

Wednesday June 10th Imaging Development - Part II
   
    2:00 – 2:30 PM  “Imaging Axon Regeneration In Vivo”
                                    Marc Hammarlund, Ph.D.
                                    Department of Genetics
                                    Yale University
    
    2:30 – 3:30 PM   Imaging the Dynamics of Embryonic Development”  
                                     Scott Fraser , Ph.D.
                                     Department of Applied Physics
                                     California Institute of Technology
    Coffee break
    4:00 – 5:00 PM    Multidimensional visualization of zebrafish embryogenesis by
                                   multidirectional selective plane illumination microscopy (mSPIM)”
  
                                     Jan Huisken , Ph.D.
                                     Department of Biochemistry and Biophysics
                                     University of California, San Francisco

------------------------

II) Classes, Hands-on Practicals, and Technical Demonstrations
   Tuesday June 9th

11:00 – 12:00     Spinning Disk Demonstration
                                   Perkin Elmer UltraView Vox demonstration TAC N239

12:00 – 1:30       " Time Correlated Single Photon Counting : use for FLIM, FRET and FCS"
                                    lecture in TAC N207

                                    followed by FLIM  demo in TAC N237

                                    Thomas Pingle , LaVision Biotec

1:00 – 2:00 PM     Laser Microdissection Demonstration
                                    Leica LMD demonstration TAC N221

1:00 – 2:00 PM     "Getting closer to reality through deconvolution: the most
                                   
payout for the photons you put in"

                                    lecture on deconvolution in TAC N211

                                    Jeff M. Reece , Reecent Technologies/ Scientific Volume Imaging

 Wednesday June 10th
    9:30 – 10:30 AM  "Choosing imaging parameters for time resolved imaging of live samples
                                    for cell tracking"
(advance registration for this lecture is strictly required!)
                                    Bill Mohler , Ph.D.
                                    Department of Genetics and Developmental Biology
                                    UConn Health Center
                                    TAC building auditorium N107

10:30–12:00 AM  Hands-on practical in small groups: 4D imaging of fluorescent Drosophila
                                    embryos with confocal or two-photon laser scanning microscopes (for group #1)
                                    TAC N221 - N229 Registration for this class is now closed. The class is full.

11:00–12:00 AM   Demo of Laser Scanning Cytometry for High Content Screening
                                    CompuCyte iCys demonstration TAC N239

1:00 – 2:00 PM    Small group break--out for post acquisition analysis using Volocity, Imaris,
                                  
or MetaMorph software (for group #1)
                                    TAC N207 - N211
Registration for this class is now closed. The class is full.

2:00 – 5:00 PM    Opportunity for individual practice - please reseve an instrument for personal use

Thursday June 11th
   10:30–12:00 AM  Hands-on practical in small groups: 4D imaging of fluorescent Drosophila
                                    embryos with confocal or two-photon laser scanning microscopes (for group #2)
                                    TAC N221 - N229 Registration for this class is now closed. The class is full.

1:00 – 2:00 PM    Small group break--out for post acquisition analysis using Volocity, Imaris,
                                   
or MetaMorph software (for group #2)
                                   TAC N207 - N211 Registration for this class is now closed. The class is full.

2:00 – 5:00 PM    Opportunity for individual practice - please reseve an instrument for personal use              

------------------------   

III) Equipment and Software Demonstrations
    Tuesday June 9th
     Software Walk-in Clinics for post acquisition image analysis
                                    Imaris (TAC N209)
9 AM - 5 PM
                                    MetaMorph (TAC N207)
1 - 5 PM
                                    Huygens (TAC N211)
9 AM - 5 PM
                                    Volocity (TAC N239)
Note: only available from 1-3 PM

9:00 – 5:00 PM     Continued access to most instrumentation throughout the day
                                    TAC N221 - N239

Wednesday June 10th
    2:00 – 5:00 PM   Software Walk-in Clinics for post acquisition image analysis
                                    Imaris (TAC N209)
9 AM - 5 PM
                                    MetaMorph (TAC N207)
9 AM - 5 PM
                                    Huygens (TAC N211)
9 AM - 3:30 PM
                                    Volocity (TAC N239)
Note: only available from 1-3 PM

9:00 –5:00 PM     Continued access to most instrumentation throughout the day
                                    TAC N2221 - N239

Thursday June 11th
    2:00 – 5:00 PM   Software Walk-in Clinics for post acquisition image analysis
                                    (Imaris is not available on this day)
                                    MetaMorph (TAC N209)
9 AM - 5 PM
                                    Huygens (TAC N211)
9 AM - 5 PM
                                    Volocity (TAC N239)
Note: only available from 1-3 PM

9:00 –5:00 PM     Continued access to most instrumentation throughout the day
                                    TAC N2221 - N239