YMW micro-course
4D imaging for cell tracking
June 10 -11th 2009
Yale School of Medicine
TAC 2nd floor teaching labs
300 Cedar St
New Haven, CT
The Yale Microscopy Workshop is a free annual event that is a combination of symposia, hands-on classes and open access to a wide variety of microscopes. A short lecture and hands-on practical “micro-course” will be offered on time resolved imaging and cell tracking with fluorescent Drosophila embryos using either two-photon laser scanning microscopes or confocal microscopes from any major vendor.
The structured learning opportunities are split into 3 separate times and places: 1) a didactic lecture on 4D imaging in small model organisms, 2) a hands-on practical in small groups at the microscopes and 3) break out sessions for small groups to perform post acquisition image analysis for cell tracking. You can attend any of these individual components or all of 3 them. If you have registered for more sessions than you can actually attend, please contact an organizer (ann.haberman@yale.edu) to release that space for someone else!
In addition to the structured class time periods, we encsourage you to spend time outside of class to try this on your own. You can reserve a microscope for use for a 1 hour time period with a vendor representative at your side. The samples we are providing are wicked cool – Drosophila embryos that are expressing a Histone-GFP fusion protein in all nuclei. (Many Thanks to Lynn Cooley's lab, and in particular Andy Hudson, for providing the flies and accessories needed to generate the embryos!)
Not really interested in Drosophila? This mirco-course will be an appropriate introduction to many of the concepts that should be applied to 4D imaging of any living sample, large or small. Vendor demonstrations on other techniques and application will be held throughout the 3 day event as well. See the schedule of events for the latest list of these demonstrations.
Wednesday June 10
9:30 – 10:30 AM "Choosing imaging parameters for time resolved imaging of live samples
for cell tracking"
Bill Mohler , Ph.D.
Department of Genetics and Developmental Biology
UConn Health Center
TAC building room N203
10:30–12:00 AM Hands-on practical in small groups: 4D imaging of fluorescent Drosophila
embryos with confocal or two-photon laser scanning microscopes (for group #1)
TAC N221 - N239
1:00 – 2:00 PM Small group break--out for post acquisition analysis using Volocity, Imaris
or MetaMorph software (for group #1) TAC N209 - N211
2:00 – 5:00 PM Opportunity for individual practice - please reseve an instrument for personal use
Thursday June 11th
10:30–12:00 AM Hands-on practical in small groups: 4D imaging of fluorescent Drosophila
embryos with confocal or two-photon laser scanning microscopes (for group #2)
TAC N221 - N239
1:00 – 2:00 PM Small group break--out for post acquisition analysis using Volocity, Imaris
or MetaMorph software (for group #2) TAC N209 - N211
2:00 – 5:00 PM Opportunity for individual practice - please reseve an instrument for personal use